Publish web server with multiple sites

I have a web server that has multiple sites.

myserver.mydomain.com/budget

myserver.mydomain.com/tracking

myserver.mydomain.com/producer

Is it possible to publish them in a single application on a single trunk?

I have tried creating a trunk,

Setting the server and public host name of the applicationl to myserver.mydomain.com

Adding the paths /budget, /tracking, /producer

But get an error either invalid URL or 500

I am using pass through authentication to the application

Thanks